15:30  24/06/2021

Investigation of Institute for research and development of organic products

1. Test duration

From April 1st to May 20th, 2021

2. Purpose

To investigate antibacterial activity of the Japanese technology multifunctional deodorant spray bottle CLODEWASH (liquid) against some pathogenic microorganisms.

3. Test microorganism

  • Pseudomonas aeruginosa ATCC 9027
  • Staphylococcus aureus ATCC 6538
  • Escherichia coli ATCC 8739
  • Salmonella typhimurium ATCC 13311
  • Candida albicans ATCC 10231
  • Mycotuberculosis ATCC 25618
  • Bacillus subtilis ATCC 6633

4. Principle of test method

A sample of the product as delivered and/or diluted with hard water (or water for ready to use products) which meet the concentration of the maufacuter recommendation. The concentration of chlorine dioxide in the test product is 25 ppm. The test microorganisms, including Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans, Bacillus subtilis, Salmonella typhimurium, Mycotuberculosis has been prepared  and mixed with the interfering substance (bovine albumin 0,3 mg/ml). The test product is added to a test suspension of bacteria in a solution of an interfering substance. 

5. Calculation of the results

  • N: is the number of bacteria per ml in the test suspension before treatment
  • Na: is the number of bacteria per ml in the test suspension after treatment and neutralization and/or submerged in peptone buffer (for hanging bag)
  • N and Na are calculated following formula: N = C/(n1 + 0,1n2)10x

Where:    Vc   is number of bacteria in each pectri disk

                 C     is the sum of Vc value taken into account

                 n1   is the number of Vc value taken into account in the lower dilution

                 n2   is the number of Vc value taken into account in the higher dilution

                 10x  is the dilution factor corresponding to the lower dilution

  • The results are shown as: 1,0-9,9 x 10x CFU/ml

Formulate a table to compare number of microorganism before and after treatment by the produts. Antibacterial capacity is measure as (N-Na)/N*100%

6. Test results of antibacterial activity

Antibacterial activity of the Japanese technology multifunctional deodorant spray bottle CLODEWASH (liquid, concentration of ClO2 is 25 ppm)     

Test microorganism Before treatment (CFU/ml) After 30 seconds (CFU/ml) After 60 seconds (CFU/ml) After 150 seconds (CFU/ml) Antibacterial capacity (%)
Pseudomonas aeruginosa 2,4 x108 <10 <10 <10 99,99
Staphylococcus aureus 1,2 x 108 <10 <10 <10 99,99
Escherichia coli 1,1 x 108 <10 <10 <10 99,99
Salmonella typhimurium 2,8 x 108 <10 <10 <10 99,99
Candida albicans 3,2 x 107 <10 <10 <10 99,99
Mycotuberculosis 2,5 x 107 <10 <10 <10 99,99
Bacillus subtilis 1,1 x 107 <10 <10 <10 99,99

Note: <10 :  Not detected

Explaination: The Japanese technology multifunctional deodorant spray bottle CLODEWASH (liquid) could kill all test microorganism with 7-8 lg reduction which is equivalent to 99,99% antibacterial capacity after 30 second treatment.

Figure 2. Time course for antibacterial activity of the Japanese technology multifunctional deodorant spray bottle CLODEWASH (liquid)

Link Attached PDF document: https://drive.google.com/file/d/1Dl_pbDsHsa1ccfGSEYJNrTdmAIvsV1FX/view?usp=sharing